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If grandparents have a doubt about the parentage of their grandchild, when the alleged parent is not available for testing for some reasons. The grandparent and grandchild test can solve the issue. We strongly recommend that both grandparents would participate in the test in order to obtain the most conclusive results. If one grandparent is not available, a grandparentage test can be done by testing only one grandparent. Child's mother's participation in test will increase the accuracy of the test. 1, Grandmother, Grandfather, and the Grandchild in the test We can use the genetic profiles of both grandparents to reconstruct the genetic profile of the alleged father. Comparing the reconstructed alleged father' genetic profile against the genetic information of the child. 2, Only One Grandparent in the test Alleged father's genetic profile cannot be reconstructed. We test the single grandparent and the grandchild to get their genetic profile and calculate their kinship index value. Kinship index value is >10, support for relationship Kinship index value is <0.1, no support for relationship Kinship index value is between 0.1 and 10, inconclusive for relationship If any sample's test fails to get valid data, we can provide you 2nd test for free. You only pay the shipping for re-collection kit